indexado en
- Abrir puerta J
- Genamics JournalSeek
- CiteFactor
- Cosmos SI
- cimago
- Directorio de publicaciones periódicas de Ulrich
- Biblioteca de revistas electrónicas
- Búsqueda de referencia
- Universidad Hamdard
- EBSCO AZ
- Directorio de indexación de resúmenes para revistas
- OCLC-WorldCat
- Convocatoria de búsqueda
- erudito
- CAMINO
- Biblioteca Virtual de Biología (vifabio)
- Publón
- Fundación de Ginebra para la Educación e Investigación Médica
- Google Académico
Enlaces útiles
Comparte esta página
Folleto de diario
Revistas de acceso abierto
- Administración de Empresas
- Agricultura y Acuicultura
- Alimentación y Nutrición
- Bioinformática y Biología de Sistemas
- Bioquímica
- Ciencia de los Materiales
- Ciencia general
- Ciencias Ambientales
- Ciencias Clínicas
- Ciencias farmacéuticas
- Ciencias Médicas
- Ciencias Veterinarias
- Enfermería y Cuidado de la Salud
- Genética y Biología Molecular
- Ingeniería
- Inmunología y Microbiología
- Neurociencia y Psicología
- Química
Abstracto
Evaluation of nested PCR targeting IS6110 of Mycobacterium tuberculosis for the diagnosis of pulmonary and extra-pulmonary tuberculosis
Shukla I, Varshney S, Sarfraz, Malik A, Ahmad Z
The purpose of this study was to compare sensitivity and specificity of the PCR from smear and culture in the diagnosis of suspected cases of pulmonary and extra pulmonary tuberculosis. This study was carried out on 140 specimens obtained from suspected cases of tuberculosis. The various specimens collected from these patients included 74 sputum, 38 endometrial biopsies, 16 CSF and 12 gastric aspirates. All the specimens were tested by ZN staining, culture was on L-J medium and PCR was performed for targeting IS6110 sequence. Out of 140 patients, 61.4% were suffering from pulmonary tuberculosis and 38.5% from extra-pulmonary tuberculosis. In these 140 patients, 40 (28.5%) were ZN smear positive for AFB, 48 (34.2%) were culture positive for AFB and 104 (74.2%) were sensitive to nested PCR for Mycobacterium tuberculosis. We observed significant difference in sensitivity of PCR for smear positive and negative cases, and also for culture positive and negative cases.