Pharmaceutica Analytica Acta

Abstracto

Liquid Chromatographic Method for Simultaneous Quantification of Alprazolam and Mebeverine in Pharmaceutical Dosage Form

Usmangani K Chhalotiya, Nishma M Patel, Dimal A Shah, Falgun A Mehta and Kashyap K Bhatt

A sensitive, rapid, accurate and reproducible Isocratic, RP-LC method was developed for determination of Alprazolam and Mebeverine in pharmaceutical dosage form. A Sunfire C18, 5 μm column with mobile phase containing methanol: buffer (0.02M KH2PO4) (70:30). The flow rate was 1.0 ml/min and effluents were monitored at 225 nm with help of PDA detector. The retention time was 6.04 min. for ALP and 3.53 min. for MEB. The linearity was in the range of 0.05-40 μg/ ml for ALP and 0.2-40 μg/ ml for MEB. The proposed method was validated with respect to linearity, accuracy, precision, specificity and robustness. Due to simplicity, rapidity and accuracy of method, believe that the method will be useful for routine quality control analysis.